a Record control number 000313391300035

a ISSN 0378-8741

a Original cataloging agency NEU GrandLibrary

a Language code of text/sound track or separate title eng

a Classification number QD31.2

b Item number .B56 2012

a Personal name Guvenc, Aysegul.

h Medium Electronic Document

a Title Biological activities of Pseudevernia furfuracea (L.) Zopf extracts and isolation of the active compounds

c Statement of responsibility, etc Aysegul Guvenc, Esra Kupeli Akkol, Ipek Suntar, Hikmet Keles, Sulhiye Yildiz, Ihsan Calis.

c Date of publication, distribution, etc 2012.

b Name of publisher, distributor, etc Elsevier,

a Summary, etc Ethnopharmacological relevance: Pseudevernia furfuracea (L.) Zopf (Parmeliaceae) is a common epiphytic lichen in the conifer-hardwood forest of Anatolia. This species is used in traditional medicine in Turkey as a treatment for wounds, eczema and hemorrhoids. Aim of the study: The present study was designed to investigate the active compounds from Pseudevernia. furfuracea, and the isolation studies yielded atraric acid (Asian et al., 2006) as the major compound and a mixture of methyl hematommate (Baumann, 1960) and methyl chlorohematommate (Bayir et al., 2006). Furthermore, methanolic extract from thalli of Pseudevernia. furfuracea and its fractions and isolates (Asian et al., 2006; Baumann, 1960; Bayir et al., 2006) were investigated for in vitro antimicrobial and antioxidant activities, and in vivo antinociceptive, anti-inflammatory and wound healing activities. Material and methods: Antimicrobial activities of the samples were determined by using the disc diffusion technique. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used as a rapid TLC screening method to evaluate the antioxidant activity of Pseudevernia. furfuracea. The thiobarbituric acid (TBA) test was used to assess the efficacy of the extracts in protecting liposomes from lipid peroxidation. In vivo inhibitory effect of the extracts on the carrageenan-induced hind paw edema model in mice was studied for the assessment of anti-inflammatory activity. p-Benzoquinone-induced abdominal constriction test was used to explore the antinociceptive effects of the extracts. Moreover, the wound healing potential of the plant extracts that were evaluated by using in vivo incision and excision wound models on rats and mice, were comparatively assessed with a reference ointment Madecassol (R). Results: Significant antimicrobial activities were observed against Gram (+) microorganisms and Candida krusei and Candida. dubliniensis in dichloromethane (DCM) and ethyl acetate (EtOAc) extracts and isolates. The methanol (MeOH), DCM and EtOAc extracts of the lichen were found to possess moderate inhibitory activity on lipid peroxidation. Methanolic extract of the lichen was found to possess significant inhibitory activity on the carrageenan-induced hind paw edema model in mice whereas the other fractions did not show any activity. While DCM and EtOAc extracts and fractions showed notable anti-inflammatory activity on carrageenan-induced hind paw edema model without inducing any apparent acute toxicity or gastric damage. Moreover, topical application of the ointment prepared with MeOH extract and EtOAc fraction onto the incised wounds exerted remarkable wound healing activity. Conclusion: The results of these experimental studies exhibited that nonpolar fractions of Pseudevernia. fiirfuracea have significant antimicrobial activity against especially Candida species and polar fractions (especially MeOH) display antioxidant, anti-inflammatory, antinociceptive and wound healing activities. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

a Topical term or geographic name as entry element Chemistry

a Topical term or geographic name as entry element Near East University Article

a Topical term or geographic name as entry element Yakın Doğu Üniversitesi Makale

a Topical term or geographic name as entry element Plant Sciences

a Topical term or geographic name as entry element Pseudevernia furfuracea

a Topical term or geographic name as entry element Parmeliaceae

g Relationship information DEC 18 2012, Vol 144 Issue 3, p726-734.

t Title Journal Of Ethnopharmacology

x International Standard Serial Number 03788741

u Uniform Resource Identifier http://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.1016/j.jep.2012.10.021